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Biosafety guidelines for sorting of unfixed cells

โœ Scribed by Ingrid Schmid; Janet K. A. Nicholson; Janis V. Giorgi; George Janossy; Annalisa Kunkl; Peter A. Lopez; Stephen Perfetto; Larry C. Seamer; Phillip N. Dean

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
407 KB
Volume
28
Category
Article
ISSN
0196-4763

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โœฆ Synopsis

The International Society of Analytical Cytology (ISAC) Biohazard Working Group presents guidelines for sorting of unfixed cells, including known biohazardous samples, using jet-in-air, deflecteddroplet cell sorters. There is a risk that personnel operating these instruments could become exposed to droplets and aerosols containing biological agents present in the samples. The following guidelines can aid in the prevention of exposures of laboratory personnel to pathogens contained in the sort samples. The document provides biosafety recommendations for sample handling, operator training and protection, laboratory facility design, and instrument setup and maintenance. In addition, it describes in detail methods for assessment of instrument aerosol containment. Recommendations provided here may also help laboratories to obtain institutional and/or regulatory agency approval for sorting of unfixed and known biohazardous samples.

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## Abstract ## Background: Cell sorting of viable biological specimens has become very prevalent in laboratories involved in basic and clinical research. As these samples can contain infectious agents, precautions to protect instrument operators and the environment from hazards arising from the us