Introduction to Electron Microscopy for Biologists

✍ Scribed by Terence D. Allen (Eds.)

• Publisher: AP
• Year: 2008
• Tongue: English
• Leaves: 531
• Series: Methods in Cell Biology 88
• Edition: 1
• Category: Library

✦ Synopsis

Introduction to Electron Microscopy for Biologists is ideal for the scientist who may be considering electron microscopy as a tool to extend molecular, biochemical, or light microscope observations to the next level of structural information, only available by electron microscopy. Each chapter briefly surveys the present state of structural information in a particular area, be it an individual but widely occurring molecule such as actin or collagen, together with the methods for visualization, either as an extracted and purified entity, or in situ within its biological context. Not only is this book an introduction to electron microscopy in general, but it is also useful for those within the field who wish to move to a different area of expertise, for instance an approach based on rapid freezing, rather than more conventional protocols. This should be a first choice reference for any biologist wanting to know 'what does it look like' across the full spectrum of cell and molecular biology of life science. Illustrates the advantages of electron microscopy and the protocols required for fine structural visualization of individual molecules upwards, ranging from mammalian tissues to experimental organisms. Comprehensive chapters cover both structural information on each area as well as the protocols themselves *Includes methods covering specific labeling, as well as methods for quantification and three dimensional reconstruction from molecular to tissue levels.

✦ Table of Contents

Content:
Contributors to Volume 88
Pages xiii-xv

Chapter 1 High Pressure Freezing and Freeze Substitution of Schizosaccharomyces pombe and Saccharomyces cerevisiae for TEM Review Article
Pages 3-17
Stephen Murray

Chapter 2 Electron Probe X-ray Microanalysis for the Study of Cell Physiology Review Article
Pages 19-43
E. Fernandez-Segura, Alice Warley

Chapter 3 Preparation of Cells and Tissues for Immuno EM Review Article
Pages 45-58
Paul Webster, Heinz Schwarz, Gareth Griffiths

Chapter 4 Quantification of Structures and Gold Labeling in Transmission Electron Microscopy Review Article
Pages 59-82
John Lucocq

Chapter 5 Combined Video Fluorescence and 3D Electron Microscopy Review Article
Pages 83-95
Alexander A. Mironov, Roman S. Polishchuk, Galina V. Beznoussenko

Chapter 6 From Live-Cell Imaging to Scanning Electron Microscopy (SEM): The Use of Green Fluorescent Protein (GFP) as a Common Label Review Article
Pages 97-108
Sheona P. Drummond, Terence D. Allen

Chapter 7 Immunolabeling for Scanning Electron Microscopy (SEM) and Field Emission SEM Review Article
Pages 109-130
Martin W. Goldberg

Chapter 8 Immunogold Labeling of Thawed Cryosections Review Article
Pages 131-149
Peter J. Peters, Jason Pierson

Chapter 9 Close-to-Native Ultrastructural Preservation by High Pressure Freezing Review Article
Pages 151-164
Dimitri Vanhecke, Werner Graber, Daniel Studer

Chapter 10 High-Pressure Freezing and Low-Temperature Fixation of Cell Monolayers Grown on Sapphire Coverslips Review Article
Pages 165-180
Siegfried Reipert, Gerhard Wiche

Chapter 11 Freeze-Fracture Cytochemistry in Cell Biology Review Article
Pages 181-204
Nicholas J. Severs, Horst Robenek

Chapter 12 Three-Dimensional Molecular Architecture of the Plasma-Membrane-Associated Cytoskeleton as Reconstructed by Freeze-Etch Electron Tomography Review Article
Pages 207-236
Nobuhiro Morone, Chieko Nakada, Yasuhiro Umemura, Jiro Usukura, Akihiro Kusumi

Chapter 13 Visualization of Dynamins Review Article
Pages 237-256
Jason A. Mears, Jenny E. Hinshaw

Chapter 14 Correlated Light and Electron Microscopy of the Cytoskeleton Review Article
Pages 257-272
Sonja Auinger, J. Victor Small

Chapter 15 Electron Microscopy of Intermediate Filaments: Teaming up with Atomic Force and Confocal Laser Scanning Microscopy Review Article
Pages 273-297
Laurent Kreplak, Karsten Richter, Ueli Aebi, Harald Herrmann

Chapter 16 Studying Microtubules by Electron Microscopy Review Article
Pages 299-317
Carolyn Moores

Chapter 17 Electron Microscopy of Collagen Fibril Structure In Vitro and In Vivo Including Three-Dimensional Reconstruction Review Article
Pages 319-345
Tobias Starborg, Yinhui Lu, Karl E. Kadler, David F. Holmes

Chapter 18 Visualization of Desmosomes in the Electron Microscope Review Article
Pages 347-366
Anthea Scothern, David Garrod

Chapter 19 A Protocol for Isolation and Visualization of Yeast Nuclei by Scanning Electron Microscopy Review Article
Pages 367-387
Stephen Murray, Elena Kiseleva

Chapter 20 Scanning Electron Microscopy of Nuclear Structure Review Article
Pages 389-409
Terence D. Allen, Sandra A. Rutherford, Stephen Murray, Sheona P. Drummond, Martin W. Goldberg, Elena Kiseleva

Chapter 21 Electron Microscopy of Lamin and the Nuclear Lamina in Caenorhabditis elegans Review Article
Pages 411-429
Merav Cohen, Rachel Santarella, Naama Wiesel, Iain Mattaj, Yosef Gruenbaum

Chapter 22 Visualization of Nuclear Organization by Ultrastructural Cytochemistry Review Article
Pages 431-449
Marco Biggiogera, Stanislav Fakan

Chapter 23 Scanning Electron Microscopy of Chromosomes Review Article
Pages 451-474
Gerhard Wanner, Elizabeth Schroeder-Reiter

Chapter 24 Infection at the Cellular Level Review Article
Pages 477-496
Christian Goosmann, Ulrike Abu Abed, Volker Brinkmann

Chapter 25 Electron Microscopy of Viruses and Virus-Cell Interactions Review Article
Pages 497-524
Peter Wild

Index
Pages 525-535

Volumes in Series
Pages 537-545