Introduction of the herpes simplex virus thymidine kinase gene into mouse cells using virus DNA or transformed cell DNA
β Scribed by Minson, A.C.; Wildy, P.; Buchan, A.; Darby, G.
- Book ID
- 122841983
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 684 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0092-8674
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π SIMILAR VOLUMES
A mouse cell line (LP1-1) was established from the murine L cells deficient in thymidine kinase (L-M(TK-] by prolonged selective culture on the hypoxanthine-aminopterine-thymidine (HAT) medium following transfection with the thymidine kinase gene of herpes simplex virus type-I (HSVTK). Southern blot
## Abstract To analyze the site of integration of the herpes simplex virus type 1 (HSVβ1) thymidine kinase (TK) gene in biochemically transformed human cells, TKβHeLaβ(BU25) cells were transformed to the TK^+^ phenotype by a cloned, 2 kbp Pvull fragment of HSVβ1 DNA. The transformed cells [HeLa(BU2