Introduction of a Foreign Gene into Zebrafish and Medaka Cells Using Adenoviral Vectors
โ Scribed by Kawasaki, Toshihiro; Saito, Kenji; Mitsui, Kaoru; Ikawa, Masahito; Yamashita, Masakane; Taniguchi, Yoshihito; Takeda, Shunichi; Mitani, Kohnosuke; Sakai, Noriyoshi
- Book ID
- 121392158
- Publisher
- Mary Ann Liebert
- Year
- 2009
- Tongue
- English
- Weight
- 287 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1545-8547
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We developed a procedure to introduce a foreign gene into fertilized eggs of medakafish (Oryzias latipes) using the particle gun method, which is one of the easiest and most reliable techniques for gene transfer. A plasmid construct with the green fluorescence protein (GFP) gene driven by the madaka