Introduction of a foreign gene into medakafish using the particle gun method

✍ Scribed by Yamauchi, Masatake ;Kinoshita, Masato ;Sasanuma, Motoe ;Tsuji, Satsuki ;Terada, Minako ;Morimyo, Mitsuoki ;Ishikawa, Yuji

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 389 KB
• Volume: 287
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/1097-010x(20000901)287:4<285::aid-jez3>3.0.co;2-4

✦ Synopsis

We developed a procedure to introduce a foreign gene into fertilized eggs of medakafish (Oryzias latipes) using the particle gun method, which is one of the easiest and most reliable techniques for gene transfer. A plasmid construct with the green fluorescence protein (GFP) gene driven by the madakafish beta-actin gene promoter was successfully introduced into eggs, and the expression of GFP was observed in 20% of the primary transfectant (chimera) fish. In addition, germ line transmission of GFP was observed in 13% of the GFP-positive primary transfectant fish. The new application described here should enable us to investigate gene expression using the fish model on a routine basis without high technical sophistication. J. Exp. Zool. 287:285-293, 2000.