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Introduction of 5′-terminal functional groups into synthetic oligonucleotides for selective immobilization

✍ Scribed by Rainer Bischoff; James M. Coull; Fred E. Regnier


Book ID
107714492
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
791 KB
Volume
164
Category
Article
ISSN
0003-2697

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A novel method for the introduction of a
✍ James M. Coull; H.Lee Weith; Rainer Bischoff 📂 Article 📅 1986 🏛 Elsevier Science 🌐 French ⚖ 290 KB

Surmaary: Reaction of the 5' hydroxyl group of a support bound synthetic oligonucleotide with the new reagent N-trifluoroacetyl-2-aminoethyl-(2-cyanoethyl)-N,N-diisopropylaminophosphoramidite yields, upon deprotection, an oligonucleotide having a 5' primary amino terminus. The amino derivatized olig