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A novel method for the introduction of an aliphatic primary amino group at the 5′ terminus of synthetic oligonucleotides

✍ Scribed by James M. Coull; H.Lee Weith; Rainer Bischoff

Book ID
104218972
Publisher
Elsevier Science
Year
1986
Tongue
French
Weight
290 KB
Volume
27
Category
Article
ISSN
0040-4039

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✦ Synopsis

Surmaary: Reaction of the 5' hydroxyl group of a support bound synthetic oligonucleotide with the new reagent N-trifluoroacetyl-2-aminoethyl-(2-cyanoethyl)-N,N-diisopropylaminophosphoramidite yields, upon deprotection, an oligonucleotide having a 5' primary amino terminus. The amino derivatized oligomer can then be specifically reacted with the N-hydroxysuccinimide ester of d-biotin to give a stable oligonucleotide-biotin adduct.

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Phosphorylating agent for the synthesis
Phosphorylating agent for the synthesis of oligonucleotide with aliphatic amino group at 5′ end
✍ Toshiki Tanaka; Shigeru Tamatsukuri; Morio Ikehara 📂 Article 📅 1987 🏛 Elsevier Science 🌐 French ⚖ 264 KB

A lipophilic phosphorylating agent was prepared and used for the synthesis of pentadeoxyribonucleotide with aminoethyl group at 5' end on a polymer support by the phosphotriester method. Chemically synthesized oligodeoxyribonucleotide with defined sequence is widely used as the primer for DNA sequen