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Intrinsic fluorescence of elongation factor Tu in its complexes with GDP and elongation factor Ts

✍ Scribed by Jameson, David M.; Gratton, Enrico; Eccleston, John F.


Book ID
127095485
Publisher
American Chemical Society
Year
1987
Tongue
English
Weight
968 KB
Volume
26
Category
Article
ISSN
0006-2960

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A simple procedure for the preparation of lo-500 mg of the Escherichia coli elongation Tu-Ts complex is described. The protocol is based on the separate purification and quantitation of EF-Tu-GDP and EF-Ts, followed by mixing of equimolar amounts of each protein and removal of the displaced GDP by d