Bulk preparation and crystallization of
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Marilyn Yoder; Clare Tores; Robin Corelli; Frances Jurnak
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Article
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1985
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Elsevier Science
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English
β 426 KB
A simple procedure for the preparation of lo-500 mg of the Escherichia coli elongation Tu-Ts complex is described. The protocol is based on the separate purification and quantitation of EF-Tu-GDP and EF-Ts, followed by mixing of equimolar amounts of each protein and removal of the displaced GDP by d