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Expression of bovine mitochondrial elongation factor Ts in Escherichia coli and characterization of the heterologous complex formed with prokaryotic elongation factor Tu

✍ Scribed by Hong Xin; Karen Leanza; Linda Lucy Spremulli


Book ID
114147990
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
450 KB
Volume
1352
Category
Article
ISSN
0167-4781

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A simple procedure for the preparation of lo-500 mg of the Escherichia coli elongation Tu-Ts complex is described. The protocol is based on the separate purification and quantitation of EF-Tu-GDP and EF-Ts, followed by mixing of equimolar amounts of each protein and removal of the displaced GDP by d