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Intramembranous particles and ripples in lipid-cytochrome c bilayers

✍ Scribed by S.K. Malhotra; S. Ross; J.P. Tewari

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
702 KB
Volume
28
Category
Article
ISSN
0009-3084

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✦ Synopsis

Vesicles made from a mixture of phospholipids (PL) are unilamellar. When such lipid vesicles are incubated with reduced cytochrome c, multilamellar vesicles (PLC) are visualized by transmission electron microscopy. Both PL and PLC vesicles show a rippling in the freezefracture replicag The rippling effect is likely to be due to the tilting of fatty acid chain~ The freeze-fractured faces of PLC vesicles also show 3-4 nm intramembranous particle~ Such particles are lacking from the comparable fractur :d faces of PL vesicles that are produced in the absence of cytochrome c. It is suggested that these intramembranous particles result from the presence of cytochrome c molecules; therefore, cytochrome c molecules may penetrate the lipid bilayer.

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