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13C Selective Polarization and Spin Diffusion in a Lipid Bilayer-Bound Polypeptide by Solid-State NMR

โœ Scribed by Fang Tian; Riqiang Fu; Timothy A. Cross

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
73 KB
Volume
139
Category
Article
ISSN
1090-7807

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โœฆ Synopsis

While (15)N solid-state NMR has proven to be very advantageous for the development of structural biological methods, (13)C spectroscopy has increased sensitivity and spectral dispersion. However, large natural abundance signals and homonuclear dipolar interactions pose significant problems. Here we have used a pair of (13)C-labeled sites in a lipid-solubilized polypeptide to show the selective polarization can be used in combination with spin diffusion to achieve simplified spectra. Both unoriented and oriented samples have been used, with the latter providing a well-resolved homonuclear dipolar splitting.

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## Abstract The complete assignment of cholesterol ^1^H and ^13^C NMR resonances in a lipid bilayer environment (L~ฮฑ~โ€dimyristoylphosphatidylcholine/cholesterol 2:1) has been obtained by a combination of 1D and 2D MAS NMR experiments: ^13^C spectral editing, geโ€HSQC, dipolar HETCOR and Jโ€based HETC