Intradomain Distances in the Regulatory Domain of the Myosin Head in Prepower and Postpower Stroke States: Fluorescence Energy Transfer †

End-to-end distance distributions of fle

End-to-end distance distributions of flexible molecules from steady state fluorescence energy transfer and quenching-induced changes in the Förster distance

✍ Ignacy Gryczynski; Wieslaw Wiczk; Michael L. Johnson; Joseph R. Lakowicz 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 660 KB

We describe a new method to recover the distribution of distances between two sites on flexible molecules, which requires only steady state measurements of fluorescence intensities. We used collisional quenching of the donor fluorescence to vary the characteristic Wrster distance (R,) for energy tra