Intracellular pH regulation in hepatocytes isolated from three teleost species

✍ Scribed by Furimsky, M.; Moon, T.W.; Perry, S.F.

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 163 KB
• Volume: 284
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/(sici)1097-010x(19990901)284:4<361::aid-jez1>3.0.co;2-5

✦ Synopsis

The mechanisms of intracellular pH (pH i ) regulation were studied in hepatocytes isolated from three species of teleost: rainbow trout (Oncorhynchus mykiss), black bullhead (Ameiurus melas) and American eel (Anguilla rostrata). Intracellular pH was monitored over time using the pH-sensitive fluorescent dye BCECF in response to acid loading under control conditions and in different experimental media containing either low Na + or Cl -concentrations, the Na + -H + exchanger blocker amiloride or the blocker of the V-type H + -ATPase, bafilomycin A 1 . In trout and bullhead hepatocytes, recovery to an intracellular acid load occurred principally by way of a Na + -dependent amiloride-sensitive Na + -H + exchanger. In eel hepatocytes, the Na + -H + exchanger did not contribute to recovery to an acid load though evidence suggests that it is present on the cell membrane and participates in the maintenance of steady-state pH i . The Vtype H + -ATPase did not participate in recovery to an acid load in any species. A Cl --HCO 3 -exchanger may play a role in recovery to an acid load in eel hepatocytes by switching off and retaining base that would normally be tonically extruded. Thus, it is clear that hepatocytes isolated from the three species are capable of regulating pH i , principally by way of a Na + -H + exchanger and a Cl --HCO 3 -exchanger, but do not exploit identical mechanisms for pH i recovery.