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Toxicity of fatty acid 18:5n3 from gymnodinium cf.mikimotoi: II. intracellular pH and K+ uptake in isolated trout hepatocytes

✍ Scribed by Barthélémy Fossat; Jacqueline Porthé-Nibelle; François Sola; Armand Masoni; Patrick Gentien; Guy Bodennec

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 77 KB
Volume: 19
Category: Article
ISSN: 0260-437X
DOI: 10.1002/(sici)1099-1263(199907/08)19:4<275::aid-jat578>3.0.co;2-b

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✦ Synopsis

Effects of octadecapentaenoic acid 18:5n3 and other related polyunsaturated fatty acids present in Gymnodinium cf. mikimotoi were tested in isolated trout hepatocytes. These exotoxins decreased intracellular pH followed by a slow recovery to initial value and alkalinization of acidic compartments, suggesting an inhibition of vacuolar H ؉ -ATPases. Moreover, addition of 18:5n3 to the extracellular medium induced a decrease of K ؉ uptake into hepatocytes as a result of Na,K-ATPase inhibition. However, high concentrations (10 ؊5 -10 ؊3 M) are necessary to induce these effects.

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Toxicity of fatty acid 18:5n3 from Gymno

Toxicity of fatty acid 18:5n3 from Gymnodinium cf.mikimotoi: I. morphological and biochemical aspects onDicentrarchus labrax gills and intestine

✍ François Sola; Armand Masoni; Barthélémy Fossat; Jacqueline Porthé-Nibelle; Patr 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 275 KB

We present evidence for the toxic effects of fatty acid 18:5n3 (octadecapentaenoic acid) in the gills and intestine of the sea bass Dicentrarchus labrax. Light microscopic observation of gills showed strong mucus production and alteration of ionocytes. The Mg-and Na,K-ATPase activities were inhibite