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Intracellular distribution of DNA topoisomerase I in fibroblasts from patients with Fanconi's anaemia

✍ Scribed by Benhard Auer; Hans-Peter Vosberg; Ursula Buhre; Helmut Klocker; Monica Hirsch-Kauffmann; Manfred Schweiger

Publisher
Springer
Year
1982
Tongue
English
Weight
388 KB
Volume
61
Category
Article
ISSN
0340-6717

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✦ Synopsis

The activity of DNA topoisomerase I(DNA nicking-closing enzyme) was analysed in cytoplasmic and nuclear extracts of six independently derived Fanconi and four normal fibroblast cell lines. In all experiments the total cellular activity was predominantly found in the nuclear extracts (88-100%). In addition, a minor proportion of the enzyme (up to 12%) was randomly present in some of the cytoplasmic fractions of both Fanconi and normal fibroblasts. These results indicate that Fanconi's anaemia is probably not due to or accompanied by a maldistribution of topoisomerase I between nuclei and cytoplasm.

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