Intestinal transplantation including anorectal segment in the rat

✍ Scribed by Flavio Henrique Ferreira Galvão; Daniel Reis Waisberg; Rodrigo Martinez De Mello Vianna; Raoni De Castro Galvão; Victor Edmund Seid; Wellington Andraus; Eleazar Chaib; Luiz Augusto Carneiro D'Albuquerque

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 220 KB
• Volume: 32
• Category: Article
• ISSN: 0738-1085
• DOI: 10.1002/micr.20958

✦ Synopsis

Transplantation of small bowel and colon has been recently advocated, aiming to prevent dehydration, reduce stoma output, and promote earlier post-transplant weaning from parental nutrition. 1 Some patients needing intestinal transplantation may also have anorectal dysfunction or a permanent colostomy, which impairs quality of life. 2 The inclusion of the anorectal segment in the intestinal graft may be an attractive improvement for such patients. In this letter, we describe a model of en bloc intestinal transplantation in the rat, including jejunum, ileum, cecum, entire colon, rectum, and the anus.

In donor operation, we performed a combined perianal and midline abdominal incision (Fig. 1A). The completely dissected anorectal segment was mobilized to inside the abdomen through the perineum, sectioning rectal vessels and pudendal nerves and preserving the inferior mesenteric artery and vein. The superior mesenteric and portal veins were separated from the pancreas by division of duodenal, splenic, and left gastric veins. The abdominal aorta was dissected from the iliac bifurcation up to the diaphragm by dividing lumbar and renal arteries and the celiac trunk (Fig. 1B). After heparinization, we tied the aorta distally to the inferior mesenteric artery and cut it near the diaphragm and after the tie to perform a long aortomesenteric conduit including the superior and inferior mesenteric arteries. The portal vein was dissected