Interrelationships between cellular respiration and cell growth in tissue culture

✍ Scribed by Danes, Betty ;Christiansen, G. S. ;Leinfelder, P. J.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1954
• Tongue: English
• Weight: 504 KB
• Volume: 43
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030430309

✦ Synopsis

The purpose of this investigation is to deiiionstrate relationships that exist betu-een cellular respiration and cell growth in tissue culture. I n previous studies (Danes and Leinfelder, '51) it has been demonstrated that cellular growth in tissue cultures is altered when respiration is inhibited by hydrogen cyanide. This dependence of certain cells 011 an aerobic metabolism was shown by the curtailment of cellular migration, the occurrence of aberrant iiuclear forms, and cytolysis in cultures exposed to varying concentrations of cyanide. Howc ~e r , in order to relate such cytological changes quantitatively to inhibition of respiration, it was necessary to develop an adequate technique for measuring the respiratory activity of tissue cultures. I n this 1al)oratory the Zeuthen ('48) ('artesian diver inicrobalance f o r weighing tissue fragments and thc Gregg ( '47) capillary niicrorespironieter were adapted into ii single apparatus for use in metabolic studies of tissue cultures (Danes, Christiansen and Leinfelder, '53). By use of this apparatus it was possible to weigh, culture, determine the respiration, arid finally iiiake n~orphological studies on the same tissue explant. l \ r ~~~i a l F111ld.

This wmircli w i s iiindc possi1)le tliroagli n grant from the Dnmon-Runyoii