Among the many blood cell separators introduced into the international market in these last few years, the Fresenius AS 104 represents an advanced and safe thrombocytapheresis machine whose dcvelopment took advantage of extensive worldwide experience with blood cell separation. Nonetheless the AS 10
International Forum. Evaluation of the single needle procedure in plateletpheresis with the fresenius as 104 blood cell separator
✍ Scribed by Dr. Rainer Moog; Norbert Müller
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 517 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0733-2459
No coin nor oath required. For personal study only.
✦ Synopsis
The aim of the present study was to evaluate the single needle (SN) procedure for plateletpheresis with the AS 104 blood cell separator. One hundred fifty-seven platelet collections with different protocols were carried out and analysed with respect to platelet yield, separation efficiency, leukocyte contamination, anticoagulant infused, and donation time. SN plateletpheresis resulted in a mean platelet yield of 2 . 6 3 . 1 x 10" with separation efficiencies of 49.2-57.4%. The mean leukocyte contamination ranged from 2.8 to 40.5 X lo6.
Parameters of product quality were investigated in 14 platelet concentrates (PC). Determination of glucose, lactate, lactate dehydrogenase (LDH), and morphology score showed good quality of the PC. 0 l9Y5 Wiley-Liss, lnc.
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In this report we analyzed sixty leukapheresis procedures on 35 patients with a new protocol for the Fresenius AS 104. Yields and efficiencies for MNC, CD 34+ cells, and CFU-GM indicate that the new protocol is able to collect large quantities of hemopoietic progenitors. Procedures were performed pr