Evaluation of a new protocol for peripheral blood stem cell collection with the Fresenius AS 104 cell separator

✍ Scribed by Giacomo Menichella; Marco Lai; Luca Pierelli; Riccardo Serafini; Mariangela Vittori; Marina Ciarli; Maria Laura Foddai; Giovanni Puglia; Heike Mitschulat; Giovanni Scambia; Giuseppe Leone; Bruno Bizzi

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 67 KB
• Volume: 12
• Category: Article
• ISSN: 0733-2459
• DOI: 10.1002/(sici)1098-1101(1997)12:2<82::aid-jca5>3.0.co;2-5

✦ Synopsis

In this report we analyzed sixty leukapheresis procedures on 35 patients with a new protocol for the Fresenius AS 104. Yields and efficiencies for MNC, CD 34+ cells, and CFU-GM indicate that the new protocol is able to collect large quantities of hemopoietic progenitors. Procedures were performed processing 8.69 +/- 2.8 liters of whole blood per apheresis and modifying 3 parameters: spillover-volume 7 ml, buffy-coat volume 11.5 ml, centrifuge speed 1,500 rpm; blood flow rate was 50 ml/min and the anticoagulant ratio was 1:12. No side effects were observed during apheresis procedures except for transient paresthesia episodes promptly resolved with the administration of calcium gluconate. Yields show a high capacity of the new program to collect on average MNC 17.28 +/- 10.85 x 10(9), CD 34+ 471 +/- 553.5 x 10(6) and CFU-GM 1278.7 +/- 1346.3 x 10(4) per procedure. Separator collection efficiency on average was 49.91 +/- 23.28% for MNC, 55.1 +/- 35.66% for CFU-GM, and 62.97 +/- 23.09% for CD 34+ cells. Particularly interesting are results for MNC yields and CD 34+ efficiency; these results make the new program advantageous or similar to the most progressive blood cell separators and capable to collect a sufficient number of progenitor cells for a graft with a mean of 1.80 +/- 0.98 procedures per patient.