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Internal ribosome entry site biology and its use in expression vectors

✍ Scribed by Encarnación Martı́nez-Salas

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
115 KB
Volume
10
Category
Article
ISSN
0958-1669

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✦ Synopsis

Internal ribosome entry sites (IRESs) are cis-acting elements that recruit the small ribosomal subunits to an internal initiator codon in the mRNA with the help of cellular trans-acting factors. The recent discovery of the IRES recognition site of the eIF4G initiation factor is beginning to shed some light into how IRES elements are recognized by the translational machinery. Additionally, the progress made in the understanding of the parameters that influence start codon selection will be instrumental in establishing the rational design of bicistronic expression vectors.

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## Abstract The extreme 5′‐proximal sequence of the hepatitis C virus (HCV) genome including the 5′ non‐coding region (5′NCR) of 341 nucleotide long and the first 30 nucleotides of the core region is highly conserved among different HCV genotypes. It contains a segment termed Internal Ribosome Entr