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Internal promoters of the rpoBC operon of Escherichia coli

✍ Scribed by Ma, Jian-Chuan ;Newman, Andrew J. ;Hayward, Richard S.

Publisher: Springer
Year: 1981
Tongue: English
Weight: 585 KB
Volume: 184
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00352538

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✦ Synopsis

Four ribosomal protein genes, rplA, rplJ, rplK and rplL form an operon in E. coli together with the genes rpoB and rpoC which encode the/~ and fi' subunits of RNA polymerase. Transcription is initiated principally at two promoters, PL1 and P1, the overall structure of the operon being (in the direction of transcription) Pcl~ rplK rplA P1 rplJ rplL rpoB rpoC. Here we describe studies of phage 2 derivatives carrying various segments of this operon, which demonstrate the existence of at least three additional weak internal promoters, and help to define their positions and strengths.

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