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Transcriptional organization of the rpsA operon of Escherichia coli

✍ Scribed by Pedersen, Steen ;Skouv, Jan ;Kajitani, Masayuki ;Ishihama, Akira

Publisher: Springer
Year: 1984
Tongue: English
Weight: 649 KB
Volume: 196
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00334105

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✦ Synopsis

Three strong and two minor rpsA promoters were found by nuclease S1 mapping, promoter cloning and in vitro transcription. The longest transcript encodes a protein, located upstream from rpsA with a molecular weight of 25,000. The identity of this protein remains to be established. The other rpsA promoters are located within the gene for this 25 K protein. The rpsA leader region including the sequence of the 25 K protein and its promoter was DNA sequenced.

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