✦ LIBER ✦

Interleukin-4 inhibits lipopolysaccharide-induced expression of prostaglandin H synthase-2 in human alveolar macrophages

✍ Scribed by Tatsutoshi Yano; Harvey A. Hopkins; Stephen L. Hemplel; Martha Monick; Gary W. Hunninghake

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 568 KB
Volume: 165
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041650110

No coin nor oath required. For personal study only.

✦ Synopsis

IL-4 also decreased expression of CD14, which is the LPS-LPS-binding protein (LBP) receptor. Down-regulation of this receptor correlated with decreased expression of PGHS-2 and release of PGEz.

MATERIALS AND METHODS

Reagents

Recombinant human IL-1p and recombinant human IL-4 were purchased from R & D Systems (Minneapolis, MN). LPS and Roswell Park Memorial Institute tissue culture medium (RPMI-1640) were purchased from Sigma Chemical Co. (St. Louis, MO). Horseradish peroxidase-conjugated donkey anti-rabbit Ig was purchased from American Life Science (Buckinghamshire, England), and endotoxin free fetal calf serum (FCS) was obtained from Hyclone Laboratories (Logan, UT). 3H arachidonic acid was from DuponVNEN (Boston, Isolation of alveolar macrophages HAMS were obtained from normal volunteers with a lifetime nonsmoking history. They had no medical illness and were not taking medications, including nonsteroidal anti-inflammatory agents. The study was approved by the Committee for Investigation Involving Human Subjects at the University of Iowa, and there MA; NET-298).

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