Interleukin-4 and interleukin-13 potentiate interleukin-1 induced secretion of interleukin-6 in human osteoblast-like cells

✍ Scribed by Carl-Johan Silfverswärd; Anders Frost; Helena Brändström; Olle Nilsson; Östen Ljunggren

• Publisher: Elsevier Science
• Year: 2004
• Tongue: English
• Weight: 516 KB
• Volume: 22
• Category: Article
• ISSN: 0736-0266
• DOI: 10.1016/j.orthres.2004.02.008

✦ Synopsis

Abstract

Formation of interleukin‐6 (IL‐6) in osteoblasts and bone marrow stromal cells is believed to regulate osteoclast recruitment. The anti‐inflammatory cytokines interleukin‐4 and −13 (IL‐4 and IL‐13) stimulate IL‐6 production in human osteoblasts. We investigated the relative potencies, and synergistic effects, between IL‐4, IL‐13 and interleukin‐1 (IL‐1) on IL‐6 formation in human osteoblast‐like cells.

Isolated human osteoblast‐like cells were incubated for 72 h in the presence of various concentrations of IL‐4, IL‐13 and IL‐1, and IL‐6 secretion was measured by ELISA. All cytokines stimulated the secretion of IL‐6. The rank order of potency was IL‐1 ≫ IL‐4 > IL‐13. There were no additive or synergistic effects between IL‐4 and IL‐13. However, co‐stimulation with IL‐1 and IL‐4 resulted in a marked synergistic effect on IL‐6 secretion. Co‐ stimulation with IL‐1 and IL‐13 gave a minor synergistic effect.

In conclusion, IL‐4/13 synergistically potentiates IL‐1 induced secretion of IL‐6 in human osteoblast‐like cells. © 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.