Interferon-γ-induced JAK2 and STAT1 signalling in a human salivary gland cell line

✍ Scribed by Ava J. Wu; Zhi Jian Chen; Elaine C. Kan; Bruce J. Baum

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 166 KB
• Volume: 173
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/(sici)1097-4652(199710)173:1<110::aid-jcp13>3.0.co;2-m

✦ Synopsis

We have used a human salivary gland cell line (HSG) as a possible in vitro model to evaluate the effects of IFN-g on human salivary gland epithelium (Wu et al., , 1996(Wu et al., , 1997)). In the present study, we examined the JAK-STAT signal-transduction pathway in IFN-g-treated HSG cells. We demonstrate that JAK2 and Stat1 are phosphorylated at tyrosine residues in a time-and concentration-dependent manner following exposure to IFN-g. In addition, we show that activation of this signalling pathway is decreased by the addition of a blocking antibody to the IFN-g receptor. The same maneuver is also able to reduce by approximately 50-70% the surface expression of two IFN-g-induced immunoregulatory molecules: HLA-DR and ICAM-1. These results demonstrate that the JAK2 and Stat1 signalling pathway is active in salivary-derived epithelial cells and may contribute to their immunopathologic destruction.