Interferon and cell division. VIII. Effect of interferon on macromolecular synthesis in L1210 cells in vitro

✍ Scribed by D. Brouty-Boyé; A. Macieira-Coelho; M. Fiszman; I. Gresser

• Publisher: John Wiley and Sons
• Year: 1973
• Tongue: French
• Weight: 642 KB
• Volume: 12
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910120126

✦ Synopsis

Abstract

Interferon inhibits multiplication of murine leukemia L1210 cells and the effect is evident 18 h after subcultivation. At this time a significant decrease in the synthesis of total RNA and protein was observed in interferon‐treated cultures compared to control cultures. Likewise, the formation of polyribosomes was inhibited in interferon‐treated cultures although no effect was observed on the formation of the ribosomal subunits. As control cells and interferon‐treated cells continued to multiply and the cultures attained cell density saturation, the differences in macromolecular synthesis and polyribosome formation between the cultures became less marked. Confidence that interferon itself was responsible for the effects observed stemmed from the use of highly purified interferon preparations and the availability, as a cell control, of a subline of L1210 cells resistant to interferon action. The possibility that a single mechanism of action is common to the antiviral effect of interferon and its effect on cell division is discussed.