in vitro effects of recombinant human interferon gamma on human mesothelioma cell lines

✍ Scribed by L. Zeng; A. Buard; L. Saint-Etienne; M. C. Jaurand; J. Bignon; I. Monnet; P. Brochard; C. Boutin; J. Fleury

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: French
• Weight: 722 KB
• Volume: 55
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910550331

✦ Synopsis

Malignant mesothelioma is a tumor arising from serous surfaces and often related to asbestos exposure. Malignant mesothelioma is resistant to various forms of therapy. Radiotherapy, surgery or chemotherapy only slightly improve prognosis. IFN-y produces complete or partial responses in stage-I patients with malignant mesothelioma. The in vitro biological effect of IFN-y on malignant mesothelioma cells remains poorly elucidated. In the present study, 32 well-characterized human mesothelioma cell lines (HMCL) were treated with r-hu IFN-y at 4 doses and cell growth was determined by a colorimetric method (MTT assay). Among the 32 HMCLs tested, I I exhibited significant cell-growth inhibition; I6 were insensitive to r-hu IFN-y, and 5 were slightly inhibited. The sensitive cell lines were strongly inhibited by r-hu IFN-7. Our results show that HMCL exhibit a large range of responses to r-hu IFN-y, some of which can be compared with those obtained in vivo in humans.