✦ LIBER ✦

Interference of cellular ferric ions with DNA extraction and the application to methods of DNA determination

✍ Scribed by Nancy E.L. Hall; David E. Axelrod

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 365 KB
Volume: 79
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90417-1

No coin nor oath required. For personal study only.

✦ Synopsis

Iron present in biological samples prevents the extraction of DNA by hot acid hydrolysis as used in both the Schneider and Ogur-Rosen procedures [Schneider, W. C. (1945) J. Biol. Chem. 161,293-303; Ogur, M., and Rosen, G. (1950)Arch. Biochem.

25,262-276.1 for DNA determination. Purified DNA, which is normally hot acid hydrolyzable, is rendered nonhydrolyzable by the presence of trace (0.1 mM) quantities of iron (III). Addition of a metal ion chelator, such as EDTA, to samples before hot acid treatment of DNA overcomes the inhibitory effect of iron, and, since iron is a normal cellular component, it is recommended as a general procedure for DNA determination.

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