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Interactions of the Products, 8-Oxo-dGMP, dGMP, and Pyrophosphate with the MutT Nucleoside Triphosphate Pyrophosphohydrolase †

✍ Scribed by Saraswat, Vibhor; Massiah, Michael A.; Lopez, Gregory; Amzel, L. Mario; Mildvan, Albert S.


Book ID
127151043
Publisher
American Chemical Society
Year
2002
Tongue
English
Weight
316 KB
Volume
41
Category
Article
ISSN
0006-2960

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✍ M.A. Massiah; V. Saraswat; H.F. Azurmendi; A.S. Mildvan 📂 Article 📅 2004 🏛 Elsevier Science 🌐 English ⚖ 335 KB

The MutT pyrophosphohydrolase from E. coli (129 residues) catalyzes the hydrolysis of nucleoside triphosphates (NTP), including 8-oxo-dGTP, by substitution at Pb, to yield NMP and pyrophosphate. The product, 8-oxo-dGMP is an unusually tight binding, slowly exchanging inhibitor with a K D ¼ 52 nM, (D