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Interactions of the EcoRV restriction endonuclease with fluorescent oligodeoxynucleotides

✍ Scribed by Symon G. Erskine; Stephen E. Halford


Book ID
116132175
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
314 KB
Volume
157
Category
Article
ISSN
0378-1119

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When E. coli cells carrying the plasmid pLG13 (coding for the newly discovered type II restriction endonuclease EcoRV) are infected with phage T3 or T7, only T7 is able to replicate normally. T3 wild-type as well as its ocr- mutants are subject to DNA restriction in vivo and in vitro. The EcoRV enzy