Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells

✍ Scribed by Aiye Liang; Xiaomian Zhou; Qiuyan Wang; Xin Liu; Jianhua Qin; Yuguang Du; Keyi Wang; Bingcheng Lin

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 300 KB
• Volume: 27
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.200500758

✦ Synopsis

Abstract

The interactions between granulocyte colony‐stimulating factor (G‐CSF) and dextran sulfate (DS) with different chain lengths and sulfate contents were studied by capillary zone electrophoresis. It was found that DS with a molecular mass of 500 kDa (DS500) could bind to G‐CSF and the binding constant and binding sites were determined using Scatchard plot to be 1.17×10^6^ M^−1^ and 3, respectively. DS with a molecular mass of 40 kDa also had the affinity to G‐CSF and the binding constant and binding sties were 1.01×10^6^ M^−1^ and 8, respectively. However, DS with a molecular mass of 8 kDa and the non‐sulfated saccharide, dextran, had no affinity to G‐CSF. The results indicate that the interactions between G‐CSF and DS are dependent on the chain lengths and sulfate contents of the saccharides. In addition, the effects of G‐CSF‐binding DS on a G‐CSF‐dependent leukemia cell line were investigated using biological methods. Results show that DS500 plus G‐CSF has potential therapeutic effect on cancers because their combination could inhibit the growth and induce the differentiation of the leukemia cells.