✦ LIBER ✦

Interactions of cationic porphyrins with double-stranded oligodeoxynucleotides: a study by electrospray ionisation mass spectrometry

✍ Scribed by Catarina I. V. Ramos; Cristina M. Barros; Ana M. Fernandes; M. G. Santana-Marques; A. J. Ferrer Correia; João P. C. Tomé; Maria do Carmo T. Carrilho; Maria Amparo F. Faustino; Augusto C. Tomé; M. G. P. M. S. Neves; J. A. S. Cavaleiro

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 420 KB
Volume: 40
Category: Article
ISSN: 1076-5174
DOI: 10.1002/jms.936

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Electrospray ionisation mass spectrometry (ESI‐MS), electrospray ionisation tandem mass spectrometry (ESI‐MS/MS) and Ultraviolet‐visible (UV–vis) spectroscopy were used to investigate the non‐covalent interactions between small oligonucleotide duplexes with the GC motif and a group of cationic meso(N‐methylpyridynium‐4‐yl)porphyrins (four free bases with one to four positive charges, and the zinc complex of the tetracationic free base).

The results obtained point to outside binding of the porphyrins, with the binding strength increasing with the number of positive charges. Fragmentations involving losses from both chains were observed for the porphyrins with N‐methylpyridinium‐4‐yl groups in opposite meso positions. Copyright © 2005 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Interaction between antitumor drugs and

Interaction between antitumor drugs and a double-stranded oligonucleotide studied by electrospray ionization mass spectrometry

✍ Gabelica, Valérie; De Pauw, Edwin; Rosu, Frédéric 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 198 KB 👁 2 views

Electrospray ionization mass spectrometry was used to investigate the complex formation between a doublestranded oligonucleotide and various antitumor drugs belonging to two categories: intercalators (ethidium bromide, amsacrine and ascididemin) and minor groove binders (Hoechst 33258, netropsin, di

Studies of non-covalent interactions of

Studies of non-covalent interactions of actinomycin D with single-stranded oligodeoxynucleotides by ion spray mass spectrometry and tandem mass spectrometry

✍ Yin Liang Hsieh; Yu-Tsyr Li; Jack D. Henion; Bruce Ganem 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 435 KB 👁 1 views

Non-covalent binding of actinomycin D (AMD) to single-stranded DNA (ssDNA) was observed by ion spray mass spectrometry. Interactions between AMD and different sequences of non-self-complementary oligodeoxynucle+ tides were investigated as a function of p R Non-covalent AMDlssDNA complexes disappeare