Interaction study of aspirin or clopidogrel on pharmacokinetics of donepezil hydrochloride in rats by HPLC-fluorescence detection

✍ Scribed by Mitsuhiro Wada; Junichiro Nishiwaki; Tomoko Yamane; Yuichi Ohwaki; Hassan Y. Aboul-Enein; Kenichiro Nakashima

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 219 KB
• Volume: 21
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.794

✦ Synopsis

Abstract

The present study aims to investigate the possibility of interaction of aspirin (Asp) or clopidogrel (CG) on donepezil (DP) hydrochloride in rats by HPLC‐fluorescence detection. The separation of DP was achieved in ca. 13 min without interference of Asp and CG on the chromatogram. DP levels in rat plasma with a single administration of DP (5 mg/kg, i.p., group I) and those with a co‐administration of Asp (200 mg/kg, p.o., group II or 200 mg/kg, i.p., group III) or CG (5 mg/kg, p.o., group IV) were monitored. The DP concentrations determined in rat plasma ranged from 25.0 to 336.1 ng/mL. Pharmacokinetic parameters for these groups were calculated and compared with one another. No significant difference was observed on the comparison of group I with other groups except for the mean resident time of group IV (p = 0.012). These basic findings may help clinical inference when DP is co‐administered with Asp and CG to human. Copyright © 2007 John Wiley & Sons, Ltd.