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Interaction of pyridoxal-5′-phosphate and acetohydroxy acid isomeroreductase

✍ Scribed by Eleanor M. Shematek; Stuart M. Arfin


Book ID
119404919
Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
208 KB
Volume
44
Category
Article
ISSN
0006-291X

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Fluorometric assay of pyridoxal and pyri
✍ M.S. Chauhan; K. Dakshinamurti 📂 Article 📅 1979 🏛 Elsevier Science 🌐 English ⚖ 517 KB

A new and very sensitive fiuorometric method for the determination of pyridoxal and pyridoxal 5'-phosphate is reported. The specificity is based on the reductive amination of pyridoxal and its Y-phosphate with methyl anthranilate and sodium cyanoborohydride at pH 4.5 to 5.0. Separation of the highly