Interaction of onconase with the human ribonuclease inhibitor protein
β Scribed by Rebecca F. Turcotte; Ronald T. Raines
- Book ID
- 116297590
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 295 KB
- Volume
- 377
- Category
- Article
- ISSN
- 0006-291X
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## Abstract Derivatives of ribonuclease A (RNase A) with modifications in positions 1 and/or 7 were prepared by subtilisincatalyzed semisynthesis starting from synthetic RNase 1β20 peptides and Sβprotein (RNase 21β124). The lysyl residue at position 1 was replaced by alanine, whereas Lysβ7 was repl
Ribonuclease A (RNase A) and the ribonuclease inhibitor protein (RI) form one of the tightest known protein-protein complexes. RNase A variants and homologues, such as G88R RNase A, that retain ribonucleolytic activity in the presence of RI are toxic to cancer cells. Herein, a new and facile assay i