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Interaction of fluorescein isothiocyanate with the (H+ + K+)-ATPase

โœ Scribed by R.J. Jackson; J. Mendlein; G. Sachs

Book ID
115726288
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
510 KB
Volume
731
Category
Article
ISSN
0005-2736

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Specific labeling is required for energy transfer measurements and to avoid artifacts in the use of fluorophores as reporter groups. Therefore, a method for specific modification by one of the most popular reagents for P-type ATPases (fluorescein 5-isothiocyanate) has been developed. Sulfhydryl reag