Interaction of cis-diamminedichloroplatinum (II) with sensitive and resistant L1210 cell lines: Drug binding to nuclei and DNA
β Scribed by Maria Foka; Jean Belehradek Jr.; Jacques Paoletti
- Book ID
- 115780668
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 829 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0006-2952
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DNA samples isolated from control nuclei and nuclei treated by cis-and trans-diamminedichloroplatinum (DDP) were analyzed by gel electrophoresis. There were no changes in Mr when DNA isolated from nuclei treated with trans-DDP was analyzed. Scans of DNA isolated from nuclei treated with cis-DDP reve
In order to study the mechanisms responsible for resistance to CDDP, 5 human tumor cell lines were made resistant to CDDP by repeated in vitro exposures. After cloning it was found that the cell lines developed were between 3.3-fold and 17-fold more resistant to CDDP than the parental cell lines at
The results presented here demonstrate that a cisdiamminedichloroplatinum(II) (DDP)-resistant human ovarian-carcinoma cell line is also cross-resistant to the spermine analogue N 1 ,N 12 -bis(ethyl)spermine (BESPM). We report that C13\* cells, which are approximately 20-fold resistant to DDP, simila