Integration of morphometry and X-ray microanalysis in ultrathin sections of biological material
β Scribed by W.C. de Bruijn
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 181 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0304-3991
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## Abstract Elemental microanalysis of biological material in the frozenβhydrated state using inβvacuum proton induced Xβray emission is described for the first time. For this purpose, a commercially available cryotransfer system was modified and coupled to the experimental chamber of the nuclear m
Standards were prepared for quantitative X-ray microanalysis of the halogen elements Cl, Br and I in sections of resin embedded biological specimens. Halogenated aromatic compounds were dissolved in resin, which subsequently was polymerized. Homogeneity and stability of the standards were determined
The analysis of bulk frozen-hydrated biological samples has developed now to a level where practical application of the technique is possible. Provided the sample is carefully coated with a conductive metal, the development of a space charge capable of causing a significant distortion of the electro