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Integration of a fluorescence detection system and a laminate-based disposable microfluidic chip

✍ Scribed by Rudi Irawan; Tjin Swee Chuan; Fu Chit Yaw

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
140 KB
Volume
45
Category
Article
ISSN
0895-2477

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✦ Synopsis

A fluorescence-detection system for immunoassay applications in a laminate-based microfluidic chip has been developed and tested. Blue-LED, plastic fiber, CCD detector, mylar-D, and fluorescein are used as an excitation source, a light coupler, a fluorescence detector, a microfluidic substrate, and a sample, respectively. The system is able to detect from 1 to 1000 g/L fluorescein. A method to avoid cross talk is also presented.

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