The use of CdTe quantum dot fluorescent microspheres in fluoro-immunoassays and a microfluidic chip system
✍ Scribed by Qiang Ma; Xinyan Wang; Yabing Li; Xingguang Su; Qinhan Jin
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 264 KB
- Volume
- 22
- Category
- Article
- ISSN
- 1522-7235
- DOI
- 10.1002/bio.982
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✦ Synopsis
Abstract
Polystyrene fluorescent microspheres prepared by deposition of CdTe quantum dots (QDs) are used in an immunoassay in this study. CdTe QDs/polyelectrolyte multilayers on the surface of polystyrene microspheres have been formed by layer‐by‐layer self‐assembly via electrostatic interactions. As a model antigen, rabbit IgG has been bound to the outermost layer of the fluorescent microspheres. The immunoreaction between fluorescent microspheres/rabbit IgG and the corresponding antibody was confirmed by change of the fluorescence spectrum and competitive immunoassay. This approach allowed detection of the antigen (rabbit IgG) in the range 1–500 mg/L, based on the change in the fluorescence intensity of the reporter (fluorescent microspheres/rabbit IgG). A novel microfluidic chip device with a laser‐induced fluorescence system was established and used for the detection of fluorescent microspheres in this study. Copyright © 2007 John Wiley & Sons, Ltd.