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Integrated amino acid sensors for detection of L-glutamate, L-lysine, L-arginine, and L-histidine

✍ Scribed by Hiroaki Suzuki; Eiichi Tamiya; Isao Karube

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 554 KB
Volume: 6
Category: Article
ISSN: 1040-0397
DOI: 10.1002/elan.1140060406

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✦ Synopsis

Using microfabricated Clark oxygen electrodes. we integrated amperometric amino acid sensors to detect L-glutamate, L-lysine, L-arginine, and L-histidine. The oxygen electrode consists of an anisotropically etched rectangular groove with a silver cathode and a Ag/AgCl anode, a Fluorinated Ethylene-Propylene (FEP) gas-permeable membrane, and a 0.1 M KCl electrolyte gel inserted after fabrication. Enzymes were immobilized on the gas-permeable membrane. L-glutamate was measured using L-glutamate oxidase. To detect L-lysine, L-arginine, and L-histidine, decarboxylases and autotrophic bacteria were used. The response times of the sensors ranged from 30 seconds to 10 minutes and a fairly linear relationship was observed between the output current of each sensor and the substrate concentration. We operated the sensors in the same buffer solution.

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