Instability of pyruvate-C14 in aqueous solution as detected by enzymic assay

✍ Scribed by Emanuel Silverstein; P.D. Boyer

• Book ID: 102625340
• Publisher: Elsevier Science
• Year: 1964
• Tongue: English
• Weight: 410 KB
• Volume: 8
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(64)90244-1

✦ Synopsis

The use of rapid ensymic methods for the estimation of radiopurity, which were essential for equilibrium reaction rate experiments (1, 2)) led to the observation of a hitherto undescribed rapid and gross deterioration of pyruvate-Cl4 in aqueous solution under commonly used conditions of handling and storage. Methods for determination of pyruvate-Cl4 radiopurity, and the description of the marked decomposition and simple methods for its prevention are described herein. Some additional details and radiopurity assessments with W-labeled NAD', NADH, lactate, acetaldehyde, ethanol, glucose, and ATP are described elsewhere (1, 2). Von Korff has also noted impurities in pyruvate-Cl4 samples from various suppliers and presented alternate methods of purification and storage (3).

Methods

Sodium pyruvate-l-Cl4 was obtained from California Biochemical Corp., Los Angeles, Calif., (1.5 mc/mmole), and from Nuclear Chicago Co., Chicago, 111. (4.8 mc/mmole) ; sodium pyruvate-2-C4

(3.6 mc/ mmole) from Nichem. Co., Bethesda, Md.