Insights into the Branched-Chain Formation of Mycarose: Methylation Catalyzed by an (S)-Adenosylmethionine-Dependent Methyltransferase

✍ Scribed by Huawei Chen; Zongbao Zhao; Tina M. Hallis; Zhihong Guo; Hung-wen Liu

• Book ID: 101364893
• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 101 KB
• Volume: 40
• Category: Article
• ISSN: 0044-8249
• DOI: 10.1002/1521-3773(20010202)40:3<607::aid-anie607>3.0.co;2-8

✦ Synopsis

A C-methyltransferase involved in methyl-branch formation in sugars has been characterized for the first time. TylC3, an (S)-adenosylmethylthionine(AdoMet)-dependent enzyme, catalyzes the attachment of a methyl branch [Eq. (1)] in the biosynthesis of L-mycarose, an unusual sugar found in tylosin and as its O-3-methyl derivative in erythromycin. The C-3 methylation proceeds with inversion of configuration and does not require the assistance of any cofactors. The turnover rate is 1.4±0.1 min . TDP=thymidine-5'-dihydrogenphosphate.