Inside Cover: Exploring the Conformation-Regulated Function of Titin Kinase by Mechanical Pump and Probe Experiments with Single Molecules (Angew. Chem. Int. Ed. 6/2010)

✍ Scribed by Elias M. Puchner; Hermann E. Gaub

• Book ID: 101572145
• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 389 KB
• Volume: 49
• Category: Article
• ISSN: 0044-8249
• DOI: 10.1002/anie.200907116

✦ Synopsis

The molecular-force sensor titin kinase (TK) is embedded in the M-band structure of the sarcomere at an ideal position to sense force imbalances. H. E. Gaub and E. M. Puchner demonstrate in their Communication on page 1147 ff. a new AFM-based single-molecule pump-and-probe protocol to mechanically prepare different conformations of TK and to read out their function. The results show that the binding pocket for the co-substrate ATP is shielded by two sequential barriers in the forceinduced activation pathway.