Drug-resistance element Tn5 coding for kanamycin resistance was used for mutagenesis of Alcaligenes eutrophus strain H16. The vehicle for introducing Tn5 into A. eutrophus was plasmid pJB4JI harboured by Escherichia coli. Kanamycin-resistant transconjugants occurred at a frequency of approximately 5
β¦ LIBER β¦
Insertional transposon mutagenesis by electroporation of released Tn5 transposition complexes
β Scribed by Reznikoff, William S.; Goryshin, Igor Y.; Jendrisak, Jerry; Hoffman, Les M.; Meis, Ronald
- Book ID
- 109827479
- Publisher
- Nature Publishing Group
- Year
- 2000
- Tongue
- English
- Weight
- 164 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1087-0156
- DOI
- 10.1038/72017
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