New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition

✍ Scribed by Way, J.C.; Davis, M.A.; Morisato, D.; Roberts, D.E.; Kleckner, N.

• Book ID: 120467110
• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 1011 KB
• Volume: 32
• Category: Article
• ISSN: 0378-1119
• DOI: 10.1016/0378-1119(84)90012-X

✦ Synopsis

We describe below several new variants of the tetracycline-resistance transposon TnlO which are more useful than the wild-type transposon for many types of genetic and physical analysis of bacteria. These derivatives have one or more of the following new properties: (i) new drug resistance markers; (ii) high transposition frequencies; (iii) removal of the transposase gene to a position outside of the transposing segment; (iv) internal deletions which eliminate the ability of TnlO to make adjacent deletion/inversions; or (v) addition of a trp-lac operon fusion segment just inside one terminus such that insertion can automatically generate a transcriptional fusion to the interrupted operon. Phage and plasmid vehicles carrying these new elements are described.