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Insertion of IS4 into the tnpA gone of Tn3: sequencing of the insertion site

โœ Scribed by Morita, Masayuki; Sugino, Yoshinobu


Book ID
123274517
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
388 KB
Volume
96
Category
Article
ISSN
0378-1119

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In vitro insertion of the ? attachment s
โœ Pastrana, Ricardo ;Brammar, William J. ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 575 KB

The region of the phage lambda chromosome containing the attachment site (P.P') and the genes int and xis, excised by the action of endonuclease R.EcoRI, has been inserted into the unique site for that enzyme on the promiscuous conjugative plasmid, RP4, generating the recombinant plasmid RP4att lamb