𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Inserted sequence in the mitochondrial 23S ribosomal RNA gene of the yeast Saccharomyces cerevisiae

✍ Scribed by Faye, G�rard ;Dennebouy, Nicole ;Kujawa, Chantal ;Jacq, Claude

Publisher
Springer
Year
1979
Tongue
English
Weight
924 KB
Volume
168
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

The sequence organization of the yeast mit-DNA region carrying the large ribosomal RNA gene and the polar locus omega was examined. Hybridization studies using rho- deletion mutants and electron microscopy of the heteroduplexes formed between 23S rRNA and the appropriate restriction fragments, lead to the conclusion that the 23S rRNA1 gene of the omega+ strains is split by an insertion sequence of 1,000-1,100 bp. In contrast, no detactable insertion was found in the 23S rRNA gene of the omega- strains. The size and the location of the insert found in the 23S rRNA gene of the omega+ strains appear to be identical to those of the sequence delta which had previously been found to characterize the difference (at the omega locus) between the mitDNA of the wild type strains carrying the omega+ or omega- alleles (Jacq et al., 1977).

📜 SIMILAR VOLUMES

Saccharomyces cerevisiae mutants defecti
Saccharomyces cerevisiae mutants defective in the maturation of ribosomal RNA
✍ Venkov, Pencho V. ;Vasileva, Angelina P. 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 709 KB

Slow-growing mutants were isolated after mutagenesis of the osmotic-sensitive strain Saccharomyces cerevisiae VY1160. The isolated mutants in rich media have generation times from 300 to 400 min at 30 degrees C. Studies on the biosynthesis of rRNAX have shown, that the processing of 37S pre-rRNA in

Leakiness of termination codons in mitoc
Leakiness of termination codons in mitochondrial mutants of the yeast Saccharomyces cerevisiae
✍ Weiss-Brummer, Brigitte ;Hüttenhofer, Alexander ;Kaudewitz, Fritz 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 814 KB

Seven mutants in exon 1 of the mitochondrial cob gene in yeast are described with respect to their translation products, RNA pattern, and deoxyribonucleotide sequence alteration(s). Sequence analysis of the mutations, which previously were shown to cause premature termination of apocytochrome b, rev