Due to the absence of repetition of the rRNA genes in S. cerevisiae mitochondria, isolation of ribosomal mutants at the level of the rRNA genes is relatively easy in this system. We describe here a novel thermosensitive mutation, ts1297, localized by rho- deletion mapping in (or very close to) the s
β¦ LIBER β¦
Genetics of mitochondrial ribosomes of yeast: Mitochondrial lethality of a double mutant carrying two mutations of the 21S ribosomal RNA gene
β Scribed by Julou, C. ;Bolotin-Fukuhara, M.
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 499 KB
- Volume
- 188
- Category
- Article
- ISSN
- 0026-8925
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The sequence organization of the yeast mit-DNA region carrying the large ribosomal RNA gene and the polar locus omega was examined. Hybridization studies using rho- deletion mutants and electron microscopy of the heteroduplexes formed between 23S rRNA and the appropriate restriction fragments, lead