Insect cell culture and baculovirus propagation in protein-free medium
β Scribed by Duane Inlow; Andrea Shauger; Brian Maiorella
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 454 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1573-0603
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π SIMILAR VOLUMES
The properties of Sf9 and Tn5 insect cells were analyzed comparatively under serum-free culture conditions. Sf9 cells in SF90011 medium apparently utilized sucrose as a primary nutrient both before and after virus infection, yielding small amounts of lactate and ammonia. Tn5 cells in Excell 401 medi
In order to develop an efficient process for large-scale production of recombinant protein, various factors were studied which affect the productivity of Sf-9 (Spodoptera frugiperda) insect cells when using the baculovirus expression system. It was shown that upon infection with the Bac-BRV6L recomb